Phau Ntawv Qhia Txog Cov Lus Qhia Pipette Sib Txawv

Cov lus qhia pipette yog dab tsi?

Pipette cov lus qhia yog cov khoom siv pov tseg rau pipettes siv los hloov cov kua kom raug. Lawv tuaj nyob rau hauv ntau qhov ntau thiab tsawg, cov ntaub ntawv, thiab hom, xws li tus qauv, qis-adhesion, lim, thiab cov lus qhia ntev ntev.

Pipette cov lus qhia yog qhov tseem ceeb hauv kev sim tshawb fawb thiab siv dav hauv kev tshawb fawb txog lub neej, chemistry, kws tshuaj, biotechnology, thiab molecular biology. Vim lawv cov kev siv sib txawv, cov tswj hwm thoob ntiaj teb tau teeb tsa cov qauv zoo los xyuas kom meej qhov tseeb thiab kev ntseeg siab hauv kev tshawb fawb. Cotaus, lub tuam txhab muaj npe nrov ntawm cov khoom siv roj ntsha hauv Suav teb, tsim cov lus qhia zoo pipette uas ISO, CE, thiab FDA tau ntawv pov thawj, ua kom ntseeg tau thiab ua raws li kev tshawb fawb tshawb fawb.

Niaj hnub no, cia peb tshawb txog ntau yam ntawm cov lus qhia pipette, kom nkag siab txog lawv cov yam ntxwv tshwj xeeb thiab cov ntawv thov hauv kev tuav cov kua dej meej.

Ntau hom pipette tswv yim

1. Standard (Universal) Pipette tswv yim

Standard pipette tswv yim, tseem hu ua universal cov lus qhia, feem ntau yog ua los ntawm high-zoo, autoclavable polypropylene. Lawv yog cov feem ntau hom pipette accessory siv nyob rau hauv lub chaw soj nstuam nrog ib tug ntau yam ntawm kev ua tau zoo yuav tsum muaj xws li los ntawm high precision mus rau reagent dispensing nrog ntau dua kam rau ua, tsim kom haum rau ntau yam pipette hom thiab qauv, ua rau lawv muaj ntau yam thiab haum rau cov kua dej. tuav haujlwm. Muaj nyob rau hauv ob qho tib si sterile thiab non-sterile versions nyob ntawm seb cov kev xav tau tshwj xeeb ntawm kev sim.

Non-Sterile vs. Sterile Tips

Cov Tswv Yim Tsis Muaj Sterile:Cov no tuaj yeem siv rau cov txheej txheem kev sim dav dav uas tsis muaj menyuam tsis taus. Lawv raug nqi zoo rau kev ua haujlwm niaj hnub lossis cov qauv tsis zoo.

Sterile Tips: Lawv yog ib qho tseem ceeb rau cov ntaub ntawv rhiab heev xws li microbiology, molecular biology, thiab kev soj ntsuam kuaj mob, raws li lawv tau pre-sterilized thiab certified dawb los ntawm cov kab mob xws li RNase, DNase, thiab endotoxins thiab lwm yam. Nws yuav zoo li txaus siab rau autoclave uas tsis yog-sterile tswv yim mus rau sterile. cov, tab sis autoclaving yuav tshem tawm txoj kev pheej hmoo ntawm kev kis kab mob los ntawm cov kab mob nyob, qhov no tsis tas txhais tau tias cov cov lus qhia yuav pub dawb RNase thiab DNase.

Yog tias koj xav tau ua cov kev ntsuam xyuas rhiab qhov twg qhov no yuav tsum tau, koj yuav tsum xaiv cov lus qhia tsis muaj menyuam yaus los ntawm cov chaw tsim khoom uas tuaj yeem lees paub tias lawv cov lus qhia tsis pub muaj RNase thiab DNase.

Cotaustxheem tswv yimtuaj nyob rau hauv ntau qhov ntau thiab tsawg (piv txwv li, 10 µL, 20 µL, 50 µL, 100 µL, 200 µL, 300 µL, 1000 µL).

2. Lim vs Non-FilterTips

Lub tswv yim lim:Cov lus qhia lim dej muaj qhov thaiv me me, feem ntau yog ua los ntawm cov khoom siv hydrophobic, nyob rau hauv lub taub. Cov lim no tiv thaiv kev sib kis ntawm cov qauv thiab cov pipette. Cov lus qhia lim feem ntau txhais tau tias siv rau hauv cov kev sim tshwj xeeb. Piv txwv li, yog hais tias tus qauv yog corrosive, volatile, los yog viscous heev nyob rau hauv cov xwm, nws muaj peev xwm ua puas lub pipette. Hauv qhov no, cov lus qhia lim tau pom zoo.

Txhua lub sij hawm koj aspirate kua, aerosols yog generated nyob rau hauv lub pipette taub. Yog tias koj tsis siv cov lus qhia lim, cov aerosols no yuav kis tau koj cov pipette thiab cov qauv tom ntej, cuam tshuam rau koj qhov kev sim. Yog li ntawd, cov lus qhia lim yog tus nqi-zoo nyob rau hauv precision thwmsim.

Cov tswv yim tsis lim:Cov lus qhia tsis yog lim dej yog cov lus qhia uas siv ntau tshaj plaws hauv cov chaw kuaj sim vim lawv tsis tshua kim dua li cov lus qhia lim. Lawv yog qhov zoo tshaj plaws rau cov qauv uas tsis yooj yim rau kev sib kis thiab tsis zoo li yuav ua rau lub pipette puas. xws li cais plasmid DNA, thiab thauj cov agarose gels, thiab lwm yam. Txawm li cas los xij, lawv tsis muaj kev sib kis-tiv thaiv cov txiaj ntsig ntawm cov lus qhia lim, ua rau lawv tsis haum rau cov kev sim tseem ceeb lossis rhiab heev.

3. Tsawg Retention vs Non-low Retention Tips (Standard)

Cov lus qhia qis tuav pipetteyog tsim tshwj xeeb los txo cov kua tuav hauv lub ntsis, kom ntseeg tau tias cov qauv hloov pauv tau zoo dua thiab zoo dua. Cov lus qhia no zoo tagnrho rau kev ua haujlwm nrog cov kua nplaum, nplaum, lossis cov kua dej muaj txiaj ntsig uas txo qis cov qauv poob yog qhov tseem ceeb. Txawm li cas los xij, lawv raug nqi ntau dua li cov lus qhia tus qauv, cov lus qhia no zoo tagnrho rau kev sau cov qauv thaum lub sijhawm PCR, protein purification, SDS-PAGE, cloning, DNA thiab RNA daim ntawv thov nrog rau ntau yam kev siv tshuaj ntsuam xyuas protein.

4. Cov Lus Qhia luv luv vs. Ntev Ntev

Cov lus qhia luv luv pipetteyog tsim los siv rau hauv ntau daim phiaj, xws li 1536 lossis 384-zoo hom, qhov twg lawv qhov me me pab lub hom phiaj nqaim kom raug. Cov lus qhia no kuj tseem txhim kho ergonomics los ntawm kev tso cov kav dej kom ze rau ntawm lub rooj ntev zaum, txo qis caj npab thaum ua haujlwm rov ua dua. Zoo tagnrho rau high-throughput kev tshuaj ntsuam thiab txhim khu kev kuaj sim.

Ntxiv qhov ntev pipette tswv yimyog ntev tshaj li cov lus qhia, muab kev tiv thaiv kab mob zoo dua los ntawm kev tso cai nkag mus rau hauv qab ntawm cov hlab ntsha thaum txo qis kev sib cuag nrog lub thawv. Cov lus qhia no zoo tagnrho rau kev siv nrog cov khoom siv hauv chaw kuaj mob xws li cov qhov dej sib sib zog nqus thiab cov raj microcentrifuge, kom ntseeg tau tias cov kua tuav tau meej hauv cov cheeb tsam nyuaj.

5. Wide-bore Pipette tswv yim

Wide-bore pipette tswv yimfeature qhov kawg ntawm qhov kawg nrog lub orifice txog li 70% loj dua li cov lus qhia tus qauv, cov yam ntxwv tshwj xeeb yog qhov tseem ceeb rau kev tshem tawm ntawm tes shearing thiab ntws tsis kam. Ua rau lawv zoo tagnrho rau kev tuav cov qauv nyuaj-rau-pipette xws li cov kab ntawm tes tsis yooj yim, genomic DNA, hepatocytes, hybridomas, thiab lwm yam kua nplaum heev. Cov lus qhia no txo ​​cov neeg kho tshuab shearing rog, tiv thaiv cell fragmentation thiab ua kom cov cell viability ntau dua thiab plating efficiency.

6. Cov lus qhia Robotic Pipette

Cov lus qhia Robotic pipetteyog tsim los rau kev siv nrog ntau yam kev siv cov tshuab ua kua dej thiab cov tshuab pipetting robots. Cov lus qhia no ua kom muaj kev sib raug zoo nrog cov npe (Hamilton, Beckman, Agilent, Tecan, thiab lwm yam) hauv kev kuaj automation, txhim kho precision thiab efficiency nyob rau hauv high-throughput daim ntaub ntawv. Robotic cov lus qhia raug tswj nyob rau hauv nruj dua kam rau ua piv rau phau ntawv qhia pipette. Cov lus qhia pib-robotic no ua kom muaj qhov tseeb, raug, thiab ua haujlwm tau zoo hauv cov ntawv thov kev lag luam thoob plaws ntau yam, suav nrog genomics, proteomics, thiab kev tshawb fawb tshuaj.

Piv txwv:

Conductive pipette tswv yimyog cov lus qhia tshwj xeeb uas siv nyob rau hauv automated pipetting systems uas yog tsim los txo electrostatic nqi buildup thaum ua kua. Cov lus qhia no yog qhov tseem ceeb rau cov ntawv thov uas muaj kev cuam tshuam electrostatic tuaj yeem cuam tshuam rau cov qauv kev ncaj ncees lossis qhov tseeb ntawm cov tshuab ua kua siv tshuab.

7. Cov Lus Qhia Tshwj Xeeb Pipette

Qee daim ntawv thov xav tau cov qauv pipette tshwj xeeb rau cov haujlwm tshwj xeeb.

Piv txwv:

PCR Cov Lus Qhia:Cov lus qhia tsim los siv rau hauv cov txheej txheem polymerase saw tshuaj tiv thaiv (PCR) los tiv thaiv kev kis kab mob los ntawm amplified DNA.
Cov Lus Qhia Cryogenic:Tshwj xeeb yog tsim los rau kev siv nrog ultra-tsawg kub thiab feem ntau tuaj nyob rau hauv ib tug robust, ruaj siv los lis cov qauv khov.

Xaus

Kev xaiv cov lus qhia pipette nyob ntawm qhov kev sim thiab hom pipette siv. Txawm hais tias nws yog rau kev tuav cov kua dej dav dav, tiv thaiv kev sib kis, lossis ua haujlwm nrog cov qauv me me lossis kim, kev nkag siab txog hom thiab cov yam ntxwv ntawm cov lus qhia pipette kom ntseeg tau tias cov kua hloov pauv tau zoo hauv chav kuaj. Ib txwm xaiv cov lus qhia uas tsim nyog rau koj qhov kev tshawb fawb tshwj xeeb los xyuas kom pom cov txiaj ntsig zoo thiab kev ntseeg tau.