Kev siv ntau ntawm ELISA Phaj

Raws li cov cuab yeej sim, cov qauv tseem ceeb ntawm covELISA phajyog ib qho ntawm cov microplates uas muaj cov khoom siv theem (xws li cov proteins thiab cov tshuaj tiv thaiv kab mob). Nyob rau hauv daim ntawv thov ntawm ELISA phaj, cov qauv yuav tsum tau soj ntsuam yuav hnov ​​​​mob nrog ib tug tshwj xeeb enzyme-labeled molecule, thiab ces ib tug pom kev hloov xim yuav raug tsim los ntawm kev ntxiv ib tug matrix substrate, thiab cov ntsiab lus los yog kev ua ntawm lub hom phiaj molecule yuav quantified. los yog ntsuas los ntawm kev kuaj pom lub teeb liab absorbance lossis fluorescence. Cov hauv qab no yog cov kev siv ntawm ELISA daim hlau hauv ntau qhov chaw:

1. Protein quantitative analysis: ELISA daim hlau tuaj yeem siv los ntsuas qhov concentration thiab kev ua haujlwm ntawm cov proteins hauv cov qauv tshuaj lom neeg xws li cov ntshav qab zib thiab cov cell supernatants, muab cov cuab yeej muaj zog rau kev kuaj pom cov qog nqaij hlav, kab mob siab kab mob, cov kab mob myocardial raug mob, thiab lwm yam. thiab pab cov kws kho mob hauv kev kuaj mob ntxov thiab tshuaj xyuas cov kab mob.

2. Kev soj ntsuam Cytokine: Hauv kev tshawb fawb txog kev tiv thaiv kab mob,ELISA daim hlautuaj yeem ntsuas cov qib cytokine hauv cell culture supernatants lossis cov kua dej hauv cov ntaub so ntswg, uas yuav pab kom nkag siab txog cov txheej txheem lom neeg xws li kev tiv thaiv kab mob thiab cov lus teb inflammatory, thiab tseem ceeb heev rau kev tsim kho tshiab thiab tshuaj.

3. Kev tshawb fawb Nucleic acid: Los ntawm ELISA daim phiaj, cov kws tshawb fawb tuaj yeem tshawb xyuas thiab txheeb xyuas cov ntsiab lus thiab kev ua haujlwm ntawm DNA lossis RNA, muab cov ntaub ntawv txhawb nqa rau kev tshawb fawb molecular biology xws li gene qhia thiab kev tswj cov noob, thiab ntxiv dag zog rau kev loj hlob ntawm thaj chaw xws li kev kho noob. thiab gene editing.

4. Kev tshawb fawb txog kev ua enzyme: ELISA daim hlau tuaj yeem ntsuas qhov kev ua haujlwm enzyme kom raug, pab cov neeg tshawb nrhiav nkag siab txog kev ua haujlwm thiab kev tswj hwm cov enzymes hauv cov kab mob, thiab muab cov ntaub ntawv tseem ceeb rau kev tshawb fawb hauv enzyme engineering, metabolic engineering thiab lwm yam haujlwm.

5. Kev tshawb fawb txog kev sib cuam tshuam Intermolecular:ELISA daim hlautuaj yeem siv tsis tau tsuas yog los ntsuas cov ntsiab lus ntawm cov molecules, tab sis kuj los kawm txog kev cuam tshuam ntawm cov molecules. Los ntawm kev sib txuas cov thev naus laus zis xws li nto plasmon resonance thiab fluorescence resonance zog hloov pauv, cov txheej txheem khi thiab kev sib cais ntawm cov molecules tuaj yeem saib xyuas lub sijhawm tiag tiag, muab cov kev xav tshiab thiab cov txheej txheem rau kev tsim tshuaj, kev sib cuam tshuam thiab lwm yam kev tshawb fawb.