Vim li cas PCR cov khoom siv feem ntau ua los ntawm PP?

"Raws li peb txhua tus paub, PCR yog ib txoj hauv kev sim hauv cov chaw sim biochemical." Cov txiaj ntsig kev sim yeej ib txwm tsis txaus siab, uas tej zaum yuav yog vim muaj kev sib kis me ntsis ntawm PCR cov khoom siv yas, lossis kev sim cuam tshuam los ntawm kev qhia txog cov inhibitors. Muaj lwm qhov laj thawj tseem ceeb: kev xaiv tsis raug ntawm cov khoom siv yuav muaj txiaj ntsig zoo rau kev sim cov txiaj ntsig.

Muaj ntau ntau yam uas cuam tshuam rau cov txiaj ntsig ntawm kev sim PCR: feem ntau muaj 7 yam hauv qab no.

1. Primers: Primers yog tus yuam sij rau cov tshuaj tiv thaiv tshwj xeeb ntawm PCR, thiab qhov tshwj xeeb ntawm cov khoom PCR yog nyob ntawm qib ntawm complementarity ntawm primers thiab cov qauv DNA;

2. Enzyme thiab nws cov concentration;

3. Qhov zoo thiab concentration ntawm dNTP;

4. Template (lub hom phiaj gene) nucleic acid;

5. Mg2+ concentration;

6. Kev teeb tsa kub thiab lub sijhawm;

7. Tus lej ntawm lub voj voog;

8. Cov khoom siv, khoom siv, thiab lwm yam.

Ntawm ntau yam cuam tshuam, cov khoom siv yog ib qho tseem ceeb heev thiab yooj yim saib xyuas.

Muaj ntau homPCR khoom siv: 8-tubes, cov raj tsis muaj ntim, cov raj txheem txheem, tsis-skirted, semi-skirted, puv-skirted, thiab ib series ntawm PCR thiab qPCR daim hlau. Nws nyuaj heev rau kev xaiv, thiab muaj ntau yam teeb meem, cia peb saib cov teeb meem uas txhua tus xaivPCR khoom siv, thiab yuav daws lawv li cas?

Yog vim li casPCR khoom sivfeem ntau ua los ntawm PP?

Teb: PCR / qPCR consumables feem ntau yog ua los ntawm polypropylene (PP), vim hais tias nws yog ib tug lom inert cov ntaub ntawv, qhov saum npoo tsis yooj yim rau adheres rau biomolecules, thiab muaj tshuaj tiv thaiv zoo thiab kub kam rau ua (yuav ua tau autoclaved ntawm 121 degrees) kab mob. thiab tuaj yeem tiv taus qhov kub thiab txias thaum caij tsheb kauj vab). Cov ntaub ntawv no feem ntau yog nyob rau hauv kev sib cuag ncaj qha nrog cov reagents los yog cov qauv, yog li cov ntaub ntawv zoo thiab cov txheej txheem ua haujlwm zoo yuav tsum raug xaiv thaum lub sijhawm tsim khoom thiab kev npaj.