Taw qhia ntawm Elisa Phaj

ELISA PLATE: Nyob rau hauv Enzyme Linked Immunosorbent Assay (ELISA), qhov purity, concentration thiab piv ntawm cov antigens, tshuaj tiv thaiv kab mob, sau cov tshuaj tiv thaiv kab mob los yog antigens koom nrog cov tshuaj tiv thaiv kab mob; Tsis yog hom, concentration thiab tej yam kev mob xws li ionic zog, pH nqi, tshuaj tiv thaiv kub thiab lub sij hawm ua lub luag hauj lwm tseem ceeb. Tsis tas li ntawd, qhov saum npoo ntawm cov khoom-theem polystyrene (Polystyrene) raws li tus neeg nqa khoom kuj tseem ua lub luag haujlwm tseem ceeb hauv kev nqus cov tshuaj tiv thaiv kab mob, tshuaj tiv thaiv kab mob lossis cov tshuaj tiv thaiv antigen-antibody complexes.

Antigens, cov tshuaj tiv thaiv kab mob thiab lwm yam biomolecules yog adsorbed rau cov neeg nqa khoom nto los ntawm ntau yam txheej txheem, suav nrog kev ua haujlwm tsis zoo los ntawm hydrophobic bonds, hydrophobic / ionic bonds, covalent bonding los ntawm kev qhia ntawm lwm pab pawg xws li amino thiab carbon pawg, thiab los ntawm kev hloov kho saum npoo av. . Hydrophilic bonding tom qab kev sib deev.

CovElisa Phajtuaj yeem muab faib ua 48-zoo thiab 96-zoo raws li tus naj npawb ntawm qhov. Qhov feem ntau siv yog 96-zoo, uas yuav tsum tau xaiv raws li koj tus nyeem ntawv microplate.

Tsis tas li ntawd, muaj cov detachable thiab tsis-detachable sawv daws yuav. Rau cov uas tsis yog-detachable, cov slats ntawm tag nrho cov board yog txuas ua ke. Tom qab ntawd, rau cov detachable sawv daws yuav, cov slats ntawm lub rooj tsavxwm yog sib cais, thiab cov sib cais boards muaj 12-qhov thiab 8-qhov strips. Feem ntau, detachable enzyme-labeled daim hlau yog feem ntau siv niaj hnub no. Yog tias koj yuav qee cov phiaj xwm ua ntej, koj tuaj yeem yuav ib co enzyme-labeled strips tam sim no.

Txawm hais tias cov microplates ua los ntawm cov tuam txhab sib txawv zoo ib yam tag nrho, qee cov ntsiab lus me me yuav txawv, xws li cov qauv, thiab lwm yam. Qhov no feem ntau yog vim lawv yuav tsum tau siv nrog cov sib txawv microplate nyeem. Yog li ntawd, thaum koj siv Thaum xaiv yuav tus nyeem ntawv microplate, koj yuav tsum xav txog seb koj tus nyeem ntawv microplate zoo li cas. Tab sis feem ntau lawv tau yoog, tsuas yog qee qhov yuav txawv. Vim tias cov khoom siv ntawm cov phaj enzyme feem ntau yog polystyrene (PS), thiab polystyrene muaj cov tshuaj lom neeg tsis zoo thiab tuaj yeem yaj los ntawm ntau yam organic solvents (xws li muaj ntxhiab hydrocarbons, halogenated hydrocarbons, thiab lwm yam), thiab yuav corroded los ntawm cov kua qaub muaj zog. thiab alkalis. , tsis tiv taus roj, thiab yooj yim hloov xim tom qab raug ultraviolet lub teeb, yog li nco ntsoov them sai sai rau cov no thaum siv covElisa Phaj.