Tuaj Txog Tshiab | SAIB | Dub Elisa Phaj

Hauv ntau qhov xwm txheej hauv kev tshawb fawb txog lub neej, kev txiav txim siab raws sij hawm, muaj txiaj ntsig thiab kev lag luam thiab kev ntsuas cov tshuaj tiv thaiv antigens lossis cov tshuaj tiv thaiv tam sim no hauv cov qauv yog qhov tseem ceeb.

Enzyme-linked immunosorbent assay (ELISA) tau ua pov thawj tias yog ib qho kev tshawb fawb tsis muaj nuj nqis thiab cov cuab yeej kuaj mob rau kev ntsuas cov tshuaj tiv thaiv kab mob lossis cov tshuaj tiv thaiv kab mob hauv cov qauv tshuaj lom neeg los ntawm adsorption ntawm paub antigens lossis cov tshuaj tiv thaiv kab mob mus rau saum npoo ntawm cov khoom thauj khoom, uas tso cai rau enzyme ( Feem ntau HRP)-labeled antigen-antibody cov tshuaj tiv thaiv ntawm cov khoom-theem nto. Cov txheej txheem no tuaj yeem siv los txheeb xyuas cov molecule antigens loj thiab cov tshuaj tiv thaiv tshwj xeeb, thiab lwm yam. Nws muaj qhov zoo ntawm kev ceev ceev, rhiab, yooj yim, thiab cov cab kuj yooj yim rau standardize. Txawm li cas los xij, qhov rhiab heev thiab muaj zog ntau yam ntawm ELISA kev tshawb pom muaj kev txwv ntau heev los ntawm qhov tsis zoo ntawm cov txheej txheem kev nqus lub teeb vim muaj kev cuam tshuam loj ntawm sab nraud ntawm cov xim hloov ntawm cov tshuaj thiab qhov tsis zoo linear ntau ntawm OD tus nqi.

DELFIA thev naus laus zis ---- yog tsuas yog hloov cov enzyme HRP nrog lanthanide chelate (Eu, Sm, Tb, Dy) daim ntawv lo ntawm cov tshuaj tiv thaiv kab mob hauv cov tshuaj ELISA ib txwm muaj. Cov lanthanides siv nyob rau hauv DELFIA yog ib chav tshwj xeeb ntawm fluorescent ntsiab, uas qhov chaw xav tau ntawm cov ntaub ntawv sim --- Elisa daim hlau. Lanthanides muaj fluorescence lifetimes ntawm microseconds los yog milliseconds, uas nyob rau hauv ua ke nrog nrog rau lub sij hawm-kho kom pom tau zoo txo ​​autofluorescence keeb kwm yav dhau los cuam tshuam, thiab lawv dav Strokes 'ua hauj lwm zoo heev txhim kho qhov rhiab heev ntawm qhov kev ntsuam xyuas.

Feem coob ntawm ELISA xaiv pob tshab enzyme labeling phaj raws li tus neeg nqa khoom thiab lub thawv, tab sis lub teeb tawm hauv cov tshuaj tiv thaiv luminescence yog isotropic, lub teeb yuav tsis tsuas yog tawg los ntawm txoj kab ntsug, tab sis kuj tawg los ntawm kab rov tav, thiab nws yuav yooj yim dhau los ntawm qhov sib txawv ntawm qhov sib txawv ntawm cov pob tshab enzyme labeling phaj thiab phab ntsa ntawm lub qhov. Cov qhov nyob sib ze sib cuam tshuam thiab cuam tshuam rau qhov kev sim.

Dawb Elisa Phaj tuaj yeem siv rau kev pom lub teeb pom kev tsis muaj zog thiab feem ntau yog siv rau kev siv tshuaj lom neeg thiab cov xim substrate (xws li dual luciferase reporter gene analysis).

Dub Dawb Elisa Phaj muaj lub teeb liab tsis muaj zog dua li daim ntawv enzyme dawb vim lawv lub teeb nqus, thiab feem ntau yog siv los kuaj pom lub teeb muaj zog, xws li kev kuaj pom fluorescence.

Qhov zoo ntawm Cotaus® Elisa Phaj

● Kev khi siab

Cotaus®Elisa daim hlau nrog lub raj dub yog ua los ntawm cov khoom siv tsis yog tus kheej-fluorescent, qhov saum npoo tau kho kom zoo heev rau nws cov protein khi lub peev xwm, uas tuaj yeem ncav cuag 500ng IgG / cm2, thiab qhov hnyav molecular ntawm lub ntsiab khi cov protein yog> 10kD .

● Tsis tshua muaj fluorescence tom qab tshem tawm cov teeb meem tshwm sim los ntawm cov tshuaj tiv thaiv tsis tshwj xeeb.

dub tubs tuaj yeem tshem tawm qee qhov tsis muaj zog tom qab cuam tshuam fluorescence vim nws yuav muaj nws lub teeb haum.

● Detachable tsim

Lub detachable tsim ntawm dawb enzyme phaj ncej thiab dub enzyme slats yog yooj yim dua rau kev khiav hauj lwm. Ua tib zoo saib xyuas qhov kev txiav txim siab disassemble, tsis txhob yuam kom tawg ntawm ib qho kawg, txwv tsis pub nws yuav yooj yim tawg.

Kev faib khoom

Yog xav paub ntxiv txog cov khoom, thov koj xav tiv tauj peb